Fig. 2. NudC downregulation by siRNA-mediated gene silencing. HeLa cells were transfected with Luc siRNA or NudC siRNA for 24-96 hours. (A) NudC protein levels were determined by immunoblotting with anti-NudC C peptide antibodies (top). Equal protein loading was determined by immunoblotting with anti-ß-actin antibodies (bottom). A representative of four independent experiments is shown. (B) The proliferation of HeLa cells transfected with either Luc siRNA or NudC siRNA, or left untransfected was determined over 96 hours. The total cell number x10⁴ in 1 ml of growth medium was determined. Identical results were obtained counting triplicate samples in two independent experiments.