Fig. 3. NudC downregulation induces multinucleation. HeLa cells were transfected with either Luc siRNA or NudC siRNA for 72 hours and stained with anti-NudC C peptide antibodies (red) and anti-ß-tubulin antibodies (green), and counterstained with DAPI (blue). (A) Luc-siRNA-transfected cells. Mitotic cells show more NudC staining (arrow). The MTOC (asterisk) is prominent in interphase cells. Cells undergoing cytokinesis are indicated (arrowhead). (B) NudC-siRNA-transfected cells. An increase in the number of large, flat cells containing multiple nuclei (arrows) and cells connected by persistent intercellular bridges (arrowheads) is seen. Bar, 10 µm. (C) Proportion of multinucleate cells after transfection with NudC siRNA or Luc siRNA, or left untransfected. Results are essentially identical in two independent experiments.