Fig. 6. NudC overexpression by adenovirus-mediated gene transfer. HeLa cells were transduced with Ad-Luc or Ad-NudC and analyzed from 24 hours to 96 hours. (A) Total cell lysates (5 µg) were immunoblotted with anti-MBP-NudC fusion protein antibodies (top) or anti-ß-actin antibodies (bottom). In the NudC blot, lanes 1-4 were exposed five times longer than lanes 5-8 in order to detect endogenous NudC expression in the Ad-Luc control cells. The faster-migrating band below NudC in lanes 6-8 might be due to degradation products that were only observed in the NudC-overexpressing cells. (B) The proliferation of HeLa cells transduced with either Ad-Luc or Ad-NudC was determined over 96 hours. Total cell number x 10⁴ in 1 ml of growth medium was determined. Identical results were obtained counting triplicate samples in two independent experiments.