Fig. 8. NudC overexpression induces cytokinesis failure and multiple centrosomes. HeLa cells transduced with Ad-NudC for 96 hours were stained with anti-ß-tubulin and counterstained with DAPI. (A,B) Abnormal midbody structures and unusual microtubule matrix are found in NudC-overexpressing cells. (A, inset) Lagging chromosomes are observed in the abnormal midzone/midbody structure. (C) Interphase cells are connected by intercellular bridge with disorganized, curving microtubules (arrows). (D) Large multinucleate cells are connected by an unusually dense midbody-like structure (inset, multiple nuclei and micronuclei in both cells). (E) Many MTOCs are detected in large multinucleate cells. (F) Multipolar spindles are observed (inset, DNA with unattached chromosomes). (G) Elongated, apolar, fragmented and curving microtubules are detected at the cell cortex (arrow). Bar, 10 µm for all panels.