Fig. 9. Plk1 is mislocalized in NudC-overexpressing cells. HeLa cells were transduced with Ad-Luc or Ad-NudC for 96 hours and cells were stained with anti-Plk1 antibodies and counterstained with DAPI as indicated. In Ad-Luc-transduced control cells, Plk1 staining is found on the centrosomes during metaphase (A) and at the midbody during cytokinesis (C). In Ad-NudC-transduced cells, Plk1 staining is punctate and irregular throughout the cell, as observed in cells that are connected by an intercellular bridge (E) and in multinucleate cells (G). (H) Multiple nuclei and micronuclei are observed by DAPI staining of DNA. Bar, 10 µm for all panels.