Fig. 1. Remodelling of mitochondria of wild-type cells upon glucose repression. Wild-type cells expressing matrix targeted GFP were grown in glycerol to log phase and transferred for 45 minutes into glucose-containing medium. (A-C) Beam-scanning confocal images of representative budding cells. Maximum intensity projections of several optical planes of the fluorescence signal overlaid with a bright field transmission image are displayed. (D-I) Confocal time-lapse microscopy recording the reorganization of the wild-type mitochondrial network upon glucose repression. Selected maximum intensity projections are displayed. The underlying data-stack comprises 165 three-dimensional stacks with 4000 single optical sections. Solid arrows indicate sites of future matrix separation. Feathered arrows indicate future fusion sites. The mitochondrial network had a morphology characteristic of cells grown in glycerol at time point zero. Bar, 2 µm. The complete dataset is available in two movies (Movie 1 and Movie 2 at jcs.biologists.org/supplemental).