Fig. 7. Effects of MTf overexpression on chondrogenic differentiation of C3H10T1/2 cells in response to ConA. C3H10T1/2 cells were transfected with MTf-expression or empty vector. (A) MTf levels in the cells transfected with the empty vector (lane 1) or MTf-expression vector (lane 2) were analyzed by immunoblotting. (B) ConA (5 µg ml^–1) was added to confluent cultures of these cells for 48 hours. (C) The proportion of spherical/polygonal/spindle-like cells among total cells was calculated. (D) The antibody mAb2 or control IgG (100 µg ml^–1) was added to confluent cultures of MTf-overexpressing C3H10T1/2 cells for 4 days. ConA (5 µg ml^–1) was added to these cultures for 48 hours. (E) The proportion of spherical/polygonal/spindle-like cells among total cells was calculated. Bar, 30 µm.