Fig. 4. Cytoplasmic accumulations of FP-SMN can be induced by expressing CFP-SMN in a GFP-SMN stable cell line. Three- dimensional projections of deconvolved serial sections of cells from line GFP-SMNE10.3 transiently transfected with a plasmid encoding CFP-SMN. An untransfected cell (A, bottom left) shows the characteristic SMN distribution of diffuse cytoplasmic signal and nuclear Cajal bodies. A transiently transfected cell (A top right, and B) shows accumulation of GFP-SMN and CFP-SMN in the cytoplasm (arrows). Note: with this combination of FP proteins, a small amount of bleed-through is seen from CFP-SMN into the GFP image, but no bleed-through is observed from GFP-SMN into the CFP channel. Bar, 10 µm.