Fig. 8. SMN and coilin co-localise in mitotic Cajal bodies (MCBs) until telophase. Three-dimensional projections of deconvolved serial sections through cells of line GFP-SMNE10.3 in metaphase (A-C), anaphase (D-F), telophase (G-I) and cytokinesis (J-L) counterstained with anti-coilin antibodies (red). GFP-SMN and coilin co-localise until late telophase (arrows in C, F and I). This association is lost soon after the re-import of coilin into the forming nuclei as the daughter cells flatten out, leaving a large number of SMN-positive bodies in the cytoplasm (arrows in L). Bar, 10 µm.