Fig. 9. Mitotic Cajal bodies (MCBs) contain Sm proteins and TMG-capped RNA. Three-dimensional projections of deconvolved serial sections through cells of line GFP-SMNE10.3 in mitosis counterstained with antibodies to Sm proteins (A-H) and TMG-capped RNA (I-L). The majority of SMN-positive bodies also contain Sm proteins and TMG-capped RNA, although some bodies contain SMN in the absence of Sm proteins (arrows in B) and some contain TMG-capped RNA in the absence of SMN (arrowheads in I). The overlays (D, H and L) show GFP-SMN in green and antibody staining in red. Bar, 10 µm.