Fig. 1. CCH promotes MT-dependent recruitment of dynein to the subapical region of acini. Lacrimal acini were fixed and processed for labeling of dynein and actin filaments, and imaged by confocal fluorescence microscopy. Dynein (green) is shown in panels A, D, G and J, actin filaments (red) in the same samples in B, E, H and K, and overlaid images in C, F, I and L. (A-C) resting acini; (D-F) CCH-stimulated acini (100 µM, 15 minutes); (G-I) nocodazole-treated acini (33 µM, 60 minutes); (J-L) nocodazole-treated acini (33 µM, 60 minutes) exposed to CCH (100 µM, 15 minutes). Asterisk, lumenal regions; arrowhead, punctate labeling. Bar, 10 µm.