Fig. 10. Dynamitin overexpression promotes increased cytoplasmic colocalization of VAMP2 and Arp1 in CCH-stimulated acini. Resting and CCH-stimulated lacrimal acini transduced with Ad-LacZ or Ad-Dynt were fixed and processed for detection of Arp1 (A-A' and B-B') and VAMP2 (C-C' and D-D'). (E-E' and F'F') Colocalization of these markers. (A-A', C-C' and E'E') Acini transduced with Ad-LacZ; (B-B', D-D' and F-F') Acini transduced with Ad-Dynt. (A-F) Resting acini; (A'-F') CCH-stimulated (100 µM, 15 minutes) acini. Bar, 10 µm. (*) Lumenal regions; arrowheads, colocalization of Arp1 and VAMP2 in the cytoplasm; arrows, colocalization of Arp1 and VAMP2 beneath the apical membrane.