Fig. 11. Dynamitin overexpression or nocodazole treatment reduce the subapical accumulation of rab3D in resting acini. Untransduced (Con) and transduced rabbit lacrimal acini were fixed and processed for detection of rab3D (green, A-C,E,F) or MTs (green, G and H) in parallel with actin filaments (red). (A,B,C) rab3D and actin filaments in untransduced, Ad-LacZ-transduced and Ad-Dynt-transduced acini, respectively. (D) rab3D distribution quantified into three categories from 366-404 lumena from 3-4 separate preparations under each condition: apical, half apical and fully dispersed. (E,F) rab3D labeling beneath actin-enriched lumenal regions in untreated and nocodazole-treated (33 µM, 60 minutes) acini, respectively. Arrowheads indicate rab3D-enriched secretory vesicles. (G,H) MTs and actin filaments in Ad-LacZ-transduced and Ad-Dynt-transduced acini, respectively. Arrows indicate MTs extending from the apical pole; asterisk, lumenal regions. Bar in F, 6 µm. Bar in H, 10 µm.