Fig. 2. CCH promotes recruitment of p150^Glued and Arp1 to the subapical region of acini. Lacrimal acini were fixed and processed for labeling of p150^Glued or Arp1 with actin filaments and imaged by confocal fluorescence microscopy. Panels A and B show p150^Glued (green) and actin filaments (red) in resting and CCH-stimulated (100 µM, 15 minutes) lacrimal acini, respectively, whereas panels C and D show Arp1 (green) and actin filaments (red) in resting and CCH-stimulated (100 µM, 15 minutes) lacrimal acini, respectively. Asterisk, lumenal regions. Bar, 10 µm.