Fig. 3. CCH increases recovery of p150^Glued and Arp1 in Triton X-100 insoluble, SDS soluble fractions. Lacrimal acini were treated without (Con) or with CCH (100 µM, 15 minutes) before sequential extraction into buffers containing saponin (Sap), Tx-100 and SDS. (A) Western blots of detergent eluates from representative preparations of Con and CCH-treated lacrimal acini showing p150^Glued, Arp1 and DIC contents in each pool. Blots used appropriate primary and IRDye^TM800-conjugated secondary antibodies. Equal volumes of each pool were loaded, with protein assays confirming that CON and CCH detergent eluates within each pool had equivalent protein contents. (B-D) Summary of changes in p150^Glued, Arp1 and DIC recovery in each eluate from Con and CCH-stimulated acini expressed as a percentage of the total cellular content of each protein. n=7-10 preparations; error bars represent s.e.m.; *Significant increase at P<0.05; ^#significant decrease at P<0.05.