Fig. 5. MT-affinity isolation of subcellular membranes from resting and CCH-stimulated acini. Lacrimal acini were incubated without (Con) and with CCH (100 µM, 15 minutes) and processed to isolate membranes using MT-affinity and release with excess ATP. Western blots using appropriate primary and IRDye^TM800-conjugated secondary antibodies from a representative preparation are shown in A, whereas B depicts summary data showing the contents of DIC and VAMP2 in MT-affinity, ATP release fractions under each condition. 40 µg of protein from resting and CCH-stimulated membrane samples were loaded under each condition. n=4-5 separate preparations. *Significant increase at P0.05.