JCS -- Wang et al. 116 (10): 2051 Figure IG7

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Fig. 6B. Dynein, dynactin and secretory and biosynthetic compartment markers after density gradient analysis of resting and CCH-stimulated acini. Lacrimal acini incubated without (resting) and with CCH (100 µM, 15 minutes) were processed by isopycnic centrifugation on sorbitol density gradients. The distributions of VAMP2, ${gamma}$ -adaptin, acid phosphatase, rab3D and rab6. Fractions were pooled for VAMP2 and ${gamma}$ -adaptin to conserve material for western blotting. Left and middle columns show distributions of markers from resting and CCH-stimulated acini, respectively, whereas the right columns show CCH-induced changes. n=3-8 preparations; error bars represent s.e.m. *P<0.05.