Fig. 1. Intracellular distribution of GFP-NLS and GFP-NLS-YA (506-696) fusion in Drosophila S2 cells. Fluorescence images of S2 cells transfected with GFP-NLS or GFP-NLS-YA (506-696). A-C shows a representative cell expressing GFP-NLS. (A) DAPI staining to mark the nucleus. (B) GFP fluorescence in nucleus and cytoplasm (indicated by *). (C) Overlay of A and B. D-G shows a representative field of cells expressing GFP-NLS-YA (506-696). (D) DAPI staining of nuclei (arrowhead indicates a transfected cell). (E) GFP fluorescence from the expressed fusion. (F) Overlay of panels D and E. (G) Higher magnification view of transfected cells expressing GFP-NLS-YA (506-696) showing GFP fluorescence in the nuclear periphery surrounding DNA (red). Bar, 10 µm.