Fig. 2. Nuclear envelope localization and targeting of GFP-NLS-YA constructs in transfected S2 cells. Confocal images of S2 cells that express the listed GFP-NLS-YA constructs. GFP fluorescence was visualized in fixed, permeabilized cells at 24 hours post-induction. The location of GFP fluorescence was determined relative to that of DNA (left panels, propidium iodide staining), lamin Dm₀ (middle panels, stained with rabbit -lamin) or actin (to visualize the cytoplasm; right panels, stained with AlexaFluor-594-coupled phalloidin). Bar, 10 µm.