Fig. 3. Summary of nuclear envelope localization and lamin interaction of C-terminal deletions of YA. Regions of the YA C-terminus (light gray box) fused in frame with the GFP coding sequence (dark gray box) and SV40 T-Ag NLS (black box) tested for nuclear periphery location (Figs 1 and 2) or in the yeast two-hybrid system (Table 1) are shown. Nuclear envelope localizations of GFP-NLS-YA fusions are: +, colocalization with lamin; –, presence of the fusion in both nucleus and cytoplasm with no preferential peripheral staining of the nucleus; `misfolded', inability of the fusion protein to enter the nucleus. Interaction of the various deletion constructs with lamin Dm₀ in the yeast two-hybrid assay: +, strong interaction; (+), weak interaction; –, no detectable interaction.