Fig. 2. Localization of UNC-60A in early C. elegans embryos. Wild-type embryos at the one-cell stage (a-c), transition from the one-cell to two-cell stage (d-f), two-cell stage (g-i), and three-fold stage (j-l) were stained by anti-UNC-60A (a,d,g,j) and anti-actin (b,e,h,k) antibodies. Merged images of double-staining of UNC-60A (red) and actin (green) are shown (c,f,i,l). Staining of DNA by DAPI (blue) is shown in c, f and i to indicate the phases in the cell cycle. The nerve ring is indicated by an arrow in j. Bar, 10 µm.