Fig. 3. Keratin 12 (K12) in corneas from wild-type and SEY (+/–) mice. K12 mRNA was detected by in situ hybridization in the corneal epithelium of wild-type (A,K) and SEY (+/–) (E,H) mice using an antisense riboprobe. A control using a sense probe is shown for a wild-type mouse (B). K12 protein was detected by immunostaining with an anti-N-terminal K12 antibody in wild-type (C) and SEY (+/–) (F,I) corneal epithelium and with an anti-C-terminal K12 antibody in SEY (+/–) (G,J). No primary antibody used (D) serves as a control. The specificity of the N-terminal K12 antibody for corneal but not conjunctival epithelium is shown in K. Abbreviations: c, corneal epithelium; cn, conjunctival epithelium.