Fig. 6. Corneal epithelial localization of ErbB2 and epidermal-growth-factor receptor (EGFR). Wild-type (A,C) and SEY (+/–) (B,D) corneas of 6-week-old mice were incubated with antibodies against ErbB2 (A,B) or EGFR (C,D). Immunostaining patterns for ErbB2 were similar in the wild-type and mutant cornea. However, there is a change in EGFR localization as revealed by a strong, diffuse staining pattern in the wild type versus the pronounced aggregation of immunoreactive product in the nuclei of SEY cells. As a control, a section of wild-type adult cornea (E) was processed exactly as above except that no primary antibody was used. Abbreviations: c, epithelium; s, stroma.