Fig. 2. Schematic representation of human filamin-2 domain structure (Thompson et al., 2000; van der Ven et al., 2000b; Xie et al., 1998), indicating the 24 Ig repeats, the actin binding domain, and the hinge region. The regions encoded by the mouse filamin-2 cDNA clones isolated in the yeast two-hybrid screen for N-RAP binding partners are shown below. Restriction enzyme analysis placed the 15 clones into seven groups of 1-6 clones each, and a total of eight of these clones were end-sequenced. The shortest mouse clones align with Ig repeats 20-24 of the human filamin-2 protein.