Fig. 5. (A,B) Purified Krp1. Duplicate loadings were used for detection (A) of purified proteins with Coomassie blue and (B) of Krp1 by immunoblot. Lanes were loaded with affinity purified GST-Krp1, and the purified Krp1 and GST following thrombin cleavage. (C,D) Krp1 binding to N-RAP recombinant proteins immobilized on Ni-NTA agarose beads. Duplicate loadings were used (C) for total protein detection with Coomassie blue and (D) for detection of bound Krp1. Significant Krp1 binding to recombinant N-RAP-SR and N-RAP-IB was observed.