Fig. 3. Fla10 protein levels and the rate of zygote formation in wild-type and
fla10 gametes. (A) Fla10 protein in the flagella of wild-type and
fla10 unactivated gametes was determined by SDS-PAGE and
immunoblotting with anti-Fla10N antibody (top). The lower panel shows the same
blot probed with an anti-LC8 antibody as a loading control. (B) Wild-type and
fla10 mt– gametes were mixed with
mt+ gametes and, at the indicated times, the extent of
zygote formation was assessed. The figure shows zygote formation as percentage
of the maximum zygote formation for each sample (70% for the wild type and 42%
for fla10). 
, wild-type; 
, fla10.