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Fig. 4. The 80 kDa isoform of CALK is mislocalized in fla10 gametes and is associated with the NP-40-insoluble, axonemal fraction of flagella. Wild-type and fla10 vegetative cells, and unactivated gametes (A) were analyzed by anti-CALK immunoblotting, as were flagella isolated from wild-type and fla10 vegetative cells and unactivated gametes (B). (C) Freely soluble, NP-40-soluble and NP-40-insoluble fractions of flagella and cell bodies isolated from unactivated fla10 gametes were analysed by anti-CALK immunoblotting.