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Fig. 1. Reduction of HS epitopes in NDST-1–/– skeletal muscle. Immunofluorescence staining for (a,c,e,g) HS-epitope AO4B05 and (b,d,f,h) acetylcholine receptor clusters in intercostal muscle of E18.5 embryos of (a,b,e,f) wildtype, (c,d) NDST-1–/–and (g,h) NDST-2–/–. Overall staining in NDST-1–/– is significantly reduced, compared to wild type and NDST-2–/– staining, except for the BL in the NMJs and their direct vicinity (n=5; see also Fig. 2a,b). Bar, 50 µm.