Fig. 3. Alteration of N- and O-sulfation levels in skeletal muscle of NDST-1^–/– mouse. The percentage of N-acetylated HS disaccharides (NAc) is strongly increased in skeletal muscle of NDST-1^–/– mice (open bars), compared to wild type (solid bars). The N-acetylated HS disaccharides fraction may contain a minor portion of chondroitin sulfate. Furthermore, the percentages of N-sulfated (NS), N-sulfated/2-O-sulfated (NS2S), and N-sulfated/6-O-sulfated/2-O-sulfated (NS6S2S) disaccharides are decreased in NDST-1^–/– mouse, whereas the percentages of 6-O-sulfated (6S), N-sulfated/6-O-sulfated (NS6S), and 2-O-sulfated/6-O-sulfated (2S6S) disaccharides are largely unaltered. (Wildtype, n=2; NDST-1^–/–, n=3).