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Fig. 2. The p368 antibody reacts with Cx43 only when it is phosphorylated at S368. Untreated (CON) or TPA-treated cells were lysed in sample buffer or treated with alkaline phosphatase (+AP lanes) prior to SDS-PAGE and immunoblotting. The blot was probed with the p368 antibody ({alpha}-p368 panel) followed by stripping and reprobing with the Cx43 antibody ({alpha}-Cx43 panel).