Fig. 5. NRK and CHO cells both show large increases in phosphorylation at S368 upon TPA treatment but the resulting Cx43 mobilities are very different. Cells were either incubated in the presence of no drugs (CON), 50 ng/ml TPA for 30 minutes (TPA) or 5 µg/ml brefeldin A for 4 hours (BFA), and processed for immunoblot and separately probed with the anti-p368 antibody (-p368) and the N-terminal Cx43 (-Cx43).