Fig. 8. G₀- and S-phase cells show different Cx43 cellular distributions and different abilities to transfer fluorescent dyes in established and junctional assembly assays. (A) G₀ cells show extensive junctional Cx43 immunostaining. (B) S-phase cells show extensive junctional and cytoplasmic membrane staining. (C) The number of cells that receive either Alexa488 (A488) or Alexa594 (A594) from the injected cell is quantitated for cells in established G₀- or S-phase cultures (mean±s.d.). (D) Cells in G₀ or S were assayed for the ability to assemble gap junctions and transfer calcein (quantitated as the number of transfers per the total number of interfaces between a calcein-loaded and a recipient cell, mean±s.d.).