Fig. 6. Differential localisation of immunoreactive Cx43-WT compared with Cx43-Y286A in response to pharmacological agents. Cells expressing either wild-type Cx43 (A) or Y286A-mutated Cx43 (B) were incubated for 3 hours in new medium either alone (control) or supplemented with 10 µM lactacystin (Lactacystin), 10 mM NH₄Cl (NH₄Cl) or 2 µg/ml Brefeldin A (BFA). The cells were then fixed, immunostained with anti-Cx43 antibodies and visualised using fluorescence microscopy. Staining at appositional membranes is indicated by arrows.