Fig. 10. RhoA activates HIF-1 and VEGF mRNA levels in normoxia. Caki-1 cells were transfected or not with vector alone (pcDNA3) or with dominant-active RhoA (pcDNA3-RhoAV14-Myc) for 24 hours using lipofectamine. Total RNA was isolated then HIF-1 (A), VEGF (B) and -tubulin (C) mRNA expressions were evaluated by RT-PCR. Two isoforms of VEGF were amplified, VEGF₁₆₅ and VEGF₁₈₉. Densitometric analysis was used to assess relative mRNA expression levels. Means ± s.e.m. from two experiments and values are plotted relative to values from normoxic cells from the sum of both isoforms is presented on the histograms. Significant differences (P<0.05) from normoxia are indicated by asterisks (*).