(Downloading may take up to 30 seconds.
If the slide opens in your browser, select File -> Save As to save it.)
Click on image to view larger version.



Fig. 3. Subcellular distribution of RhoA and Cdc42 proteins during hypoxia. After hypoxic treatment, renal cell carcinoma were fractionated into soluble and crude membrane fractions by centrifugation at 100,000 g for 1 hour. Each fraction (20 µg of protein) was separated by SDS-PAGE then immunodetected using Cdc42 (A) and RhoA antibodies (B). Densitometric analysis of both GTPases was carried out for soluble ({square}) and membrane ({blacksquare}) fractions. Means ± s.e.m. are shown from three independent experiments relative to samples from normoxic cells. Significant differences (P<0.05) from normoxia are indicated by asterisks (*).