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Fig. 8. Effect of DPI on overexpression of Rho GTPases in hypoxic conditions. Caki-1 cells were pre-treated with 10 µM DPI in normoxia. Cells were then incubated in hypoxic conditions from 0 to 4 hours. Cell lysates (20 µg of protein) were separated by SDS-PAGE, followed by immunodetection. Densitometric analysis of Cdc42 (A) and RhoA (B) expression are presented for cells in hypoxia ({blacksquare}) and cells in hypoxia plus DPI ({square}). Means ± s.e.m. for three independent experiments are indicated. Data are expressed relative to normoxic values. Significant differences (P<0.05) from normoxia are indicated by asterisks (*).