Fig. 7. Archvillin localizations in 50MB-1 myoblasts. Confocal sections of human 50MB-1 myoblasts showing -H340 signals within nuclei (A-C), in association with actin filament bundles near the basal plasma membrane (D-I) and as punctae on basal membrane surfaces (J-L). Double-label immunofluorescence of archvillin (A,D,G,J) with anti-lamin A/C (B), Alexa^TM 594-labeled phalloidin (E,H) and anti-vinculin (K). Composite images (C,F,I,L) were generated by superimposition of the -H340 signals in green and lamin (C), F-actin (F,I) and vinculin (L) signals in red; areas of overlap appear yellow. Arrows denote localizations at the ends of actin filaments (G-I) and occasional sites of colocalization with vinculin at the basal membrane (J-L). Bar, 20 µm.