Fig. 7. Fluorescent microscopy micrographs of untreated L929 fibroblasts (a); L929 fibroblasts incubated for 3 hours at 37°C in the presence of 2.5 µg/ml nocodazole (b); L929 fibroblasts incubated for 3 hours at 37°C in the presence of 2.5 µg/ml nocodazole and subsequently treated with 50 µM DLPC (<2% of endogenous phospholipids) for 3 minutes (c); cells incubated for 3 hours at 37°C in the presence of nocodazole 2.5 µg/ml and 100 nM wortmannin for 20 minutes at 37°C, and then treated with DLPC 50 µM for 3 minutes (d). Cells were fixed, permeabilized and reacted with 0.5 µM phalloidine-rhodamine (F-actin labelling). Bars, 10 µm.