Fig. 2. Lentiviral vectors allow for over-expression of connexins in primary ß-cells. (A) Immunofluorescence labelling of monolayer cultures showed that uninfected primary rat ß-cells expressed little Cx36 but no detectable Cx32 and Cx43. (B) After transduction with a lentiviral vector coding for one Cx isoform, Cx32, Cx43 and Cx36 were easily detected at the cell membrane. Some infected cells also showed a cytoplasmic labelling of variable intensity. The red background is due to the Evan's blue counterstaining of the cultures. Bar, 10 µm.