Fig. 2. Distribution of cPLA₂- compared with Golgi and ER markers. Cells were stimulated with 5 µM A23187 (in HEPES/Tyrode's buffer) in the presence of 1 mM extracellular Ca²⁺ for 1 minute. Cells were then incubated with goat polyclonal anti-cPLA₂- followed by AlexaFluor488-conjugated anti-goat antibody with either rhodamine-conjugated WGA (A) or rhodamine-conjugated ConA (B). Cells were visualized using immunofluorescence microscopy. Bar, 10 µm.