Fig. 3. Distribution of cPLA₂- compared with markers for the ER-Golgi intermediate compartment and the cis-Golgi cisternae. Cells were stimulated with 5 µM A23187 (in HEPES/Tyrode's buffer) in the presence of 1 mM extracellular Ca²⁺ for 1 minute. Cells were then incubated with goat polyclonal anti-cPLA₂-, with either mouse monoclonal anti-ERGIC53 (A) or mouse monoclonal anti-ß-COP (B) followed by anti-goat AlexaFluor488 and anti-mouse AlexaFluor594 secondary antibodies. Cells were visualized using immunofluorescence microscopy. Bar, 10 µm.