Fig. 7. (A) Distribution of cPLA₂- compared with COX-1 and COX-2. Cells were grown on coverslips and stimulated with 5 µM A23187 (in HEPES/Tyrode's buffer) in the presence of 1 mM extracellular Ca²⁺ for 1 minute. Cells were then incubated with goat polyclonal anti-cPLA₂-, with either mouse monoclonal COX-1 (A) or mouse monoclonal COX-2 (B) followed by anti-goat AlexaFluor488 and anti-mouse AlexaFluor594 secondary antibodies. Bar, 10 µm. (C) Quantification of the degree of cPLA₂- overlap with COX-1 and COX-2. Percentage overlap was calculated as described in the Materials and Methods. The graph represents results obtained from four independent experiments (±s.d.).