Fig. 6. SKPa protein is found in the cytoplasm and nucleus. (A) Western analysis of extracts from 4-8-hours-old embryos or newly hatched skpA⁺ or skpA^– larvae with SKPa antisera. Approximately 30 larvae or 100 µl of embryos were used to make each extract. Relative loading was determined from a coomassie-stained gel prepared with the same samples. (B-D) Localization of SKPa in CNS and salivary gland cells. (B) Clones of skpA^– cells generated using FLP-mediated mitotic recombination were positively marked with GFP using the MARCM system, and stained with SKPa antisera. Only background staining is detected in skpA^– cells. (C) SKPa is found in the cytoplasm and nucleus of CNS cells, and fails to co-localize with centrosomes (cnn), DNA (TOTO-3) or mitotic chromosomes (arrowhead). SKPa is partially concentrated in the nucleus of some cells (arrow). (D) SKPa is found exclusively in the nucleus of salivary gland cells. B and D are single confocal sections, C is a projection of 5 µm of confocal sections. Bar, 30 µm (B,D) or 5 µm (C).