Fig. 4. Peripherin particles and squiggles move rapidly and bi-directionally. Analyses of the motile properties of particles and squiggles were made in GFP-peripherin-transfected cells in DM for periods of 2-4 hours (A-F) and at 48 hours (G,H). Observations were restricted to the peripheral regions of cell bodies and the central domain of growth cones at later time points, as both particles and squiggles were most obvious within these regions. A-E are derived from a time-lapse series (1 frame every 5 seconds) in the region of the cell body indicated by the box in the phase image (E). The particle marked * moved in a retrograde direction, and that marked with the arrowhead moved in an anterograde direction. F-H are diagrammatic representations of the trajectories of individual peripherin squiggles in the cell bodies (F,G) and in a growth cone (H). Black dots represent the beginning of squiggle tracks. A-D, Bar, 2 µm; E-H, Bar, 5 µm.