Fig. 7. Individual peripherin structures can associate with both motors. GFP-peripherin-transfected PC12 cells were plated in DM for 4 hours and then processed for indirect immunofluorescence using anti-kinesin heavy chain (red) and dynein heavy chain (blue). It was determined that the majority of particles and squiggles associate with both kinesin and dynein (A,B,C, see asterisk in F). Some of these peripherin structures associate only with dynein (A,C,E, see arrow in F), others associate only with kinesin (A,B,D, see filled arrowhead in F), and a small percentage do not appear to associate with either motor (see open arrowhead). Bar, 5 µm.