Fig. 8. Ultrastructural analysis of peripherin particles within growth cones. PC12 cells plated in DM for 4 hours were processed for platinum replica immunogold electron microscopy using a rabbit polyclonal peripherin antibody, a mouse monoclonal kinesin heavy chain antibody and/or a mouse monoclonal dynein intermediate chain antibody. Secondary antibodies were 10 nm gold-conjugated anti-rabbit and 18 nm gold-conjugated anti-mouse antibodies. In the central domain of the growth cone, many particles associated with MT and their associated motors. A-C demonstrate kinesin and peripherin association. C is a color overlay showing peripherin (green), kinesin (pink) and MT (yellow). D-F show an association between peripherin and dynein. F is a color overlay showing peripherin (green), dynein (pink) and MT (yellow). A and D, Bar, 600 nm; B,C,E,F, Bar, 100 nm.