Fig. 2. The A1PiZ degradation rate is slowed in the add mutants. Pulse-chase radiolabeling experiments were performed with add mutants and the parent wild type, BY4742, expressing A1PiZ. (A) A1PiZ was immunoprecipitated from the cell extracts at 0, 20, 40 and 60 minutes and resolved on a 10% SDS-PAGE (Methods and Materials). (B) The relative amounts of A1PiZ were determined using the Bio-Rad Phosphor Analyses program with the amount of A1PiZ at the zero time point set at 100%. Results shown are the average of five independent experiments, ±s.d.