Fig. 3. Complementation of the add phenotype. Representative immunoassays performed as described in Materials and Methods. Deletion mutants (add06, add37, add39, add66, add67 and add68) expressing A1PiZ were transformed with expression vectors carrying the corresponding ADD gene (right) or with the appropriate vector without gene insert (left) and duplicate colonies were assayed. Standard controls were BY4742 WT parent stain expressing A1Pi (M), mutant A1PiZ (Z) or the vector with no gene insertion (0).