Fig. 1. Immunofluorescent detection of surface A2 on HUVECs. HUVECs grown on gelatin-coated glass coverslips were treated for 5 minutes with either thrombin or TRAP and allowed to recover for 1 hour. The cells were then simultaneously stained under native conditions for annexin 2 (A2; red) and the intracellular marker vimentin (Vmn; green) followed by fixation. The number and position of individual cells were determined by detection of nuclei using DAPI (blue).