Fig. 3. Detection of A2 and p11 in permeabilized HUVECs. HUVECs were treated with thrombin or TRAP for 5 minutes at 37°C, washed once with culture media supplemented with 2 mM Ca²⁺, and allowed to recover for 1 hour at 37°C. After recovery, cells grown on glass coverslips (A) were detergent permeabilized, fixed and stained for A2, p11 or vimentin (Vmn) and DAPI, whereas those grown in multiwell plates (B) were subjected to western blot analysis.